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. 2012 Oct 29;45(6):566–576. doi: 10.1111/j.1365-2184.2012.00848.x

Figure 4.

Figure 4

Analysis of the expression of osteoclast‐related genes and M‐CSF and RANKL. PBMC cultures (either maintained in BM or M+R) and PBMC + hBMC co‐cultures, were performed in the absence (control) or presence of Equisetum arvense extract, 0.004 mg/ml (a). RT‐PCR products subjected to densitometric analysis and normalized with value obtained for GAPDH. Quantification of M‐CSF and RANKL present in culture medium of co‐cultures maintained in absence or presence of 0.004 mg/ml Equisetum arvense extract (b). BM, base medium; M+R, M‐CSF + RANKL. *Significantly different from control cultures.