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Generation of a stable cell line inducibly overexpressing hCLP 46. (a) Proteins from five 293TRex‐hCLP46 cell clones incubated in absence or presence of 0.5 μg/ml Tet, were subjected to immunoblotting analyses to detect exogenous hCLP46, using an anti‐Myc antibody. Protein level of exogenous hCLP46 (b) and mRNA level of hCLP46 (c) were assayed in clone 1 after 24 and 48 h of Tet induction.
