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Effects of hCLP 46 on CDKI signalling were mediated through the Notch pathway. (a) 293TRex‐hCLP46 cells were incubated for 42 h in absence or presence of 0.5 μg/ml Tet, then treated with PBS or 5 mm EDTA. After treatment, cells were cultured for additional 6 h and analysed by immunoblotting with indicated antibodies. (b) Immunoblot band intensities were quantified against loading controls. *P ≤ 0.05.
