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. 2019 May 1;15:1744806919843046. doi: 10.1177/1744806919843046

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© The Author(s) 2019

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Graphs illustrating the effect of i.t. administration of D-serine (500 nmol) on total NO production and phosphorylation (Ser847) of nNOS in the spinal cord dorsal horn of naïve mice. (a) Exogenous D-serine significantly increased total NO concentration (measured indirectly as the concentration of its stable decomposition product nitrate) in the lumbar spinal cord dorsal horn at the 30 min post-injection time point and this increase was suppressed by pretreatment with the D-serine degrading enzyme, DAAO (0.1 U). n = 4 mice/group. (b) Results of Western blot analysis showed that the ratio of pnNOS to nNOS was decreased in the spinal cord dorsal horn following D-serine injection and that this decrease was completely blocked by pretreatment with DAAO (0.1 U). n = 6 mice/group. *P < 0.05 vs. VEH + VEH-treated group; ^#P < 0.05 vs. VEH + D-ser-treated group. VEH: vehicle; DAAO: D-amino acid oxidase; nNOS: neuronal nitric oxide synthase; pnNOS: phosphorylation of nNOS; D-ser: D-serine.