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. 2007 Jan 15;40(1):50–63. doi: 10.1111/j.1365-2184.2007.00413.x

Figure 2.

Figure 2

Pharmacodynamics of cyclosporin before (EO) and 2 h (E2) after dosing measured by expression of cytokines (part a), proliferation (PCNA) and surface antigens (part b). T‐cell function measured by levels of expression of intracellular cytokines (IFN‐γ, TNF‐α, IL‐2, IL‐4) and by expression of proliferation marker (PCNA) and surface antigens (CD25, CD95, CD134) of 45 HTx recipients before (E0) and 2 h (E2) after intake of the morning cyclosporin dose. Collected blood was either stimulated with PMA plus IONO for detection of intracellular cytokine expression, or with Con A for detection of PCNA and surface antigens. After an incubation period of 5 h for PMA, plus IONO assay or for 3 days for the Con A assay, harvested blood was analysed by multi‐colour flow cytometry. Statistically significant differences (MW rank sum test) between expression of intracellular cytokines, cell proliferation and surface antigen expression at E0 and E2 are indicated (*).