Figure 5.

HEK 293 Cells are Sensitive to Thapsigargin Early During G1. Cells were released from synchronization and plated on fresh DMEM with 10% FBS, as described in ‘Materials and Methods’. Cells were treated with 1 µm TG and 5 m m EGTA for 30 min at the times indicated. The media was then replaced and cells were allowed to progress through the cell cycle. Treated and untreated cells were fixed and processed for flow cytometry at 12, 24 and 30 h after release from mitotic arrest.