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Thapsigargin Treatment Affects The Activity and Expression of G1/S Phase Molecular Regulators. HEK 293 cells were lysed at indicated times after release from nocodazole arrest, as described in ‘Materials and Methods’. Samples were then subjected to SDS‐PAGE and Western blotting. (20 µg of lysate/per lane). Blots were probed with the following antibodies: antiphospho‐MAPK, anti‐MAPK, anti‐cyclin D1, anti‐cyclin E.
