Table 1.
Strains and plasmids used in this study
| Genotype | Source | |
|---|---|---|
| E. coli | ||
| Strain | ||
| RZ4500 | MG1655 lacZΔ145 | Ref. 18 |
| W3110hscA2-cat | L. E. Vickery, personal communication | |
| PK6307 | RZ4500 λiscS′-lacZ | Transduction of RZ4500 with λiscS′-lacZ |
| PK6311 | RZ4500 ΔiscS∷KnR, zff-208∷Tn10 | P1 transduction of Tn10 from CAG18481 (21) into PK4331 (5) |
| PK6361 | RZ4500 λPiscRiscS′-lacZ | Transduction of RZ4500 with λPiscRiscS′-lacZ |
| PK5956 | BW25113/pKD46 ΔiscR∷KnR | Transformation of BW25113/pKD46 (20) |
| PK6367 | PK6361 ΔiscR∷KnR | P1 transduction from PK5956 |
| PK6259 | RZ4500 λPiscRiscSUA-lacZ | Transduction of RZ4500 with λPiscRiscSUA-lacZ |
| PK6271 | PK6259 ΔiscR∷KnR | P1 transduction from PK5956 |
| PK6364 | RZ4500 λiscR′-lacZ | Transduction of RZ4500 with λiscR′-lacZ |
| PK6390 | PK6364 ΔiscR∷KnR | P1 transduction from PK5956 |
| PK6391 | PK6364 ΔiscS∷KnR, zff-208∷Tn10 | P1 transduction from PK6311 |
| PK6389 | PK6364 hscA2-cat | P1 transduction from W3110hscA2-cat |
| PK4855 | MG1655 ΔiscR∷KnR | P1 transduction from PK5956 |
| PK4854 | MG1655 ΔiscR | Resolution of Kn cartridge with pCP20 |
| Plasmids | ||
| pPK5960 | StuI-EcoRV 985-bp iscR fragment cloned into pACYC184, CmR | This study |
| pPK6161 | +1 to +489 iscR fragment cloned into pET-11a, ApR | This study |
| pPK6511 | −161 to +38 iscR promoter fragment cloned into pUC19-spf′, ApR | This study |