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. 2015 Jan 21;48(2):249–258. doi: 10.1111/cpr.12161

Figure 3.

Figure 3

The effect of DVL1 on the accumulation and nuclear translocation of β‐catenin. (a–d) Western blotting for β‐catenin in (a) A2780 cells transfected with pcDNA3.1‐DVL1 or pcDNA3.1 for 72 h, (b) A2780/Taxol cells treated with or without 100 μm compound 3289‐8625 for 72 h, (c) A2780/Taxol cells transfected with siControl, siDVL1 or siDVL1 plus pcDNA3.1‐DVL1 for 72 h, and (d) A2780 cells transfected with siControl and A2780/Taxol cells transfected with siControl or siDVL1 for 72 h. GAPDH was used as the internal control. Left panels show representative Western blots, and right panels show the relative quantification after normalization to GAPDH. In (a, c and d), DVL1 blots were included to confirm either the overexpression of DVL1 from pcDNA3.1‐DVL1 or the silencing of DVL1 by siDVL1. Each experiment was performed in triplicate. Δ P > 0.05; *P < 0.05. (e) Immunofluorescence analysis of the cellular distribution of β‐catenin in the cytoplasm and nucleus of A2780 and A2780/Taxol cells transfected with siControl or siDVL for 72 h. Green, β‐catenin; blue, nuclear DNA (Scale bar = 20 μm).