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. Author manuscript; available in PMC: 2019 May 2.
Published in final edited form as: Genes Cells. 2017 Aug 8;22(9):770–784. doi: 10.1111/gtc.12516

Figure 1-. Experimental pipeline and manual phenotype analysis.

Figure 1-

A Experimental pipeline. 854 out of 941 C. elegans TFs (91%) were knocked down individually by RNAi. Each experiment contained paired vector-fed animals (negative controls). Phenotypes including egg laying deficient (Egl), sterile (Ste), larval arrest (Lva), and protruding vulva (Pvl) were noted manually. For computational imaging, body size and fat content were quantified by IPPOME. All experiments were conducted blindly. Each TF was tested in three independent experiments, each containing on average 17 individual animals.

B Summary of all visually recorded phenotypes. Details of the corresponding RNAi to the TFs that causes these phenotypes are available in Dataset EV2.

C Example images of fat content phenotypes. Low levels of ORO staining upon sbp-1 (top left) knockdown and high levels of ORO staining in nhr-86 (top right) knockdown are shown compared to matched vector only negative controls.