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. 2015 May 15;21(7):558–567. doi: 10.1111/cns.12402

Figure 2.

Figure 2

Silencing of Kv1.3 in neural progenitor cells (NPCs) by shRNA interference induced increased differentiation efficiency, consistent with Psora‐4 treatment. (A) Example images of NPCs infected with shRNA lentiviruses with red fluorescence after flow cytometric sorting. The top panel shows the light field, and the bottom panel shows the red fluorescence in the same field. The cells were plated on Matrigel‐coated slides. (B) Kv1.3 mRNA expression levels in NPCs with shRNA lentivirus. Relative Kv1.3 mRNA expression was quantified and normalized to the Nonsense control group (n = 4). Nonsense control: NPCs infected with lentivirus containing the nonsense shRNA‐sequence. Blank vector: NPCs infected with empty lentivirus vector. (C) and (D) Differentiation efficiency of NPC cell lines with Kv1.3‐shRNA toward neuron lineage or astrocyte lineage. The data were calculated at Day 5 in differentiation condition (n = 4). **P < 0.01, *P < 0.05.