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. 2015 May 15;21(7):558–567. doi: 10.1111/cns.12402

Figure 3.

Figure 3

Newborn neurons derived from neural progenitor cells (NPCs) in the presence of Psora‐4 (10 nM) developed more mature morphology. (A) Immunocytochemistry images of NPC offspring with or without Psora‐4 at days 5, 10, and 14. (B) Psora‐4 influenced the morphology of newborn neurons. Left, percentage of neurons with multiple neurites (n = 3). Right, average neurite length (n = 4). At least 150 neurons were measured using ImageJ in each independent experiment. (C) Mature‐like and immature‐like neurons at Day 5 of NPC neuronal differentiation. (a) Low‐power image of mature‐like neurons with long and extensive dendrites (orange arrow) and immature‐like neurons with thin and sparse dendrites (white arrow). (b) High‐power image of boxed region in panel a. (c) Low‐power image of neurons (green) and astrocytes (red). This shows the same field as the image in panel a. (d) High‐power image of the boxed region in panel c. (D) Psora‐4 increased the percentage of mature neuron candidates at days 5, 10, and 14 in differentiation condition. (n = 5). (E) MgTX (5 nM) increased the percentage of mature‐like neurons at Day 5 (n = 3). ***P < 0.001, **P < 0.01, *P < 0.05.