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Immunoblotting of conditioned supernatants obtained from conventional in vitro culture of (a) viable EL‐4 cells, (b) an apoptotic (non‐adherent) subpopulation of the EL‐4 cell culture, (c) a subpopulation of live (adherent) EL‐4 cells, and (d) EL‐4 cells destroyed by freezing/thawing. An electophoretogram and a blot developed using anti‐HSP70 antibodies are presented for each sample.
