Fig. 4. (A) Absorption spectra of DC324 in a variety of solvents and electron density difference plots for the two observed major absorption bands S0 → S1 (low energy) and S0 → S2 (high energy) obtained from TD-DFT calculations at the M06-D3-BJ/def2-TZVP level of theory. (B) Emission spectra of DC324 in a variety of solvents. (C) Two-photon absorption spectra of DC324 and DC473 in toluene. (D) HaCaT keratinocytes treated with 10 μM DC324 for 30 min, prior to irradiation. (E) HaCaT keratinocytes treated with 10 μM DC324 after multiple short irradiations with 405 nm, 12 hours after initial imaging.
