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. 2008 Jan 16;41(1):36–58. doi: 10.1111/j.1365-2184.2007.00507.x

Figure 1.

Figure 1

Characterization of MSCs. Example of MSCs morphology at passage 1, day 15 (a) that was altered at passage 8, day 99 (b). Expression of mesodermal antigens in MSCs at passage 2 as revealed by immunofluorescence [vimentin (c), fibronectin (d), ASMA (e), laminin (f), n = 3 each]. Staining of extracellular calcium matrix deposition by von Kossa (g, h) and alizarin red (j) colorations in MSCs after osseous differentiation. Accumulation of intracytoplasmic lipid‐rich droplets revealed by oil red O staining (l, m) after adipocyte differentiation of MSCs. Respective controls are provided with undifferentiated MSCs (i, k, n). Example of representative immunophenotype of MSCs at passage 2 (o). Analyzed epitopes are indicated in the histograms. Bars indicate the fluorescence level of corresponding isotype. Pictures magnifications are: ×100 (b, g), ×200 (j), ×300 (c–f) and ×400 (a, i, k–n).