Table 1.
Primers used for RT‐PCR characterization of mesenchymal‐derived hepatocyte‐like cells
| Primer | Sequence | Size (bp) | Cycle |
|---|---|---|---|
| Albumin | S: 5′‐CCTTGGTGTTGATTGCCTTTGCTC‐3′ AS: 5′‐ CATCACATCAACCTCTGGTCTCACC‐3′ | 308 | 94 °C/1 min; 55 °C/1 min; 72 °C/2 min |
| G6Pase | S: 5′‐TCAGCTCAGGTGGTCCTCTT‐3′ AS: 5′‐CCTCCTTAGGCAGCCTTCTT‐3′ | 291 | 94 °C/1 min; 55 °C/1 min; 72 °C/2 min |
| CK‐8 | S: 5′‐AAGGGCTGACCGACGAGATC‐3′ AS: 5′‐GATCGCCACCTACAGGAAGC‐3′ | 537 | 94 °C/30 s; 50 °C/30 s; 72 °C/60 s |
| CK18 | S: 5′‐CCATGCGCCAGTCTGTGGAG‐3′ AS: 5′‐AGCAGATTGAGGAGAGCACCAC‐3′ | 322 | 94 °C/30 s; 65 °C/45 s; 72 °C/45 s |
| α1‐AT | S: 5′‐TCGCTACAGCCTTTGCAATG‐3′ AS: 5′‐TTGAGGGTACGGAGGAGTTCC‐3′ | 142 | 94 °C/30 s; 55 °C/1 min; 72 °C/2 min |
| αFP | S: 5′‐TGAAATGACTCCAGTAAACCC‐3′ AS: 5′‐AATGAGAAACTCTTGCTTCATC‐3′ | 199 | 94 °C/30 s; 55 °C/1 min; 72 °C/2 min |
| CYP 3A4 | S: 5′‐TGCTGTCTCCAACCTTCACC‐3′ AS: 5′‐GGTGGTGATGATTCCAAGCTA‐3′ | 802 | 94 °C/120 s; 50 °C/60 s; 72 °C/60 s |
| CYP 2B6 | S: 5′‐CCTCTTCCAGTCCATTACCG‐3′ AS: 5′‐GCCATACACAGAGGCAGTCA‐3′ | 551 | 94 °C/120 s; 50 °C/60 s; 72 °C/60 s |
| TAT | S: 5′‐TGAGCAGTCTGTCCACTGCCT‐3′ AS: 5′‐CTCAGATCCTCCTCATTCACAT‐3′ | 358 | 94 °C/40 s; 56 °C/50 s; 72 °C 60 s |
| TDO | S: 5′‐ATACAGAGCACTTCAGGGAGC‐3′ AS: 5′‐GATACCGAAGATGAACCCAACCA‐3′ | 316 | 94 °C/40 s; 56 °C/50 s; 72 °C/60 s |
| HNF4 | S: 5′‐CCAAGTACATCCCAGCTTTC‐3′ AS: 5′‐CTTTGACCCAGATGCCAA‐3′ | 295 | 94 °C/40 s; 56 °C/50 s; 72 °C/60 s |
| c‐met | S: 5′‐CAATGTGAGATGTCTCCAGC‐3′ AS: 5′‐TCTGCCTGCAATCTACAAGG‐3′ | 560 | 94 °C/40 s; 55 °C/40 s; 72 °C 60 S |
| PEPCK | S: 5′‐TTAGATGGGACAAAGCCTG‐3′ AS: 5′‐GCAAGACGGTGATTGTAACTC‐3′ | 226 | 94 °C/40 s; 55 °C/40 s; 72 °C/60 s |
| Vimentin | S: 5′‐CTACATCGACAAGGTGCGCTT‐3′ AS: 5′‐TGCCAGAGACGCATTGTCAA ‐3′ | 301 | 94 °C/30 s; 58 °C/30 s; 72 °C/60 s |
| ASMA | S: 5′‐ATGTGTGAAGAAGAGGACAG‐3′ AS: 5′‐ACTCGTAACTCTTCTCAAGG‐3′ | 730 | 94 °C/30 s; 55 °C/30 s; 72 °C/30 s |
| Fibronectin | S: 5′‐ACGGGAGCCTCGAAGAGCAA‐3′ AS: 5′‐TGCAAGTGATGCGTCCGTG‐3′ | 607 | 94 °C/30 s; 55 °C/30 s; 72 °C/30 s |
| GAPDH | S: 5′‐CGGAGTCAACGGATTTGGTCGTAT‐3′ AS: 5′‐AGC CTT CTC CAT GGT GGT‐3′ | 307 | 94 °C/1 min; 58 °C/1 min; 72 °C/2 min |
α1‐AT, α1‐antitrypsin; αFP, α‐fetoprotein; ASMA, α‐smooth muscle actin; CK, cytokeratin; CYP, cytochrome P450; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; G6Pase, glucose‐6‐phosphatase; HNF4, hepatic nuclear factor 4; PEPCK, phosphoenolpyruvate carboxykinase; TAT, tyrosin aminotransferase; TDO, tryptophan‐2,3‐dioxygenase.