Table 3.
BM samples | |||||||
---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | ||
BM data | BM donor age | 21 years | 47 years | 8 years | 34 years | 54 years | 28 years |
Passage | P1 | P2 | P1 | P3 | P3 | P2 | |
Growth | 34 PD | 23 PD | 31 PD | 28 PD | 29 PD | 26 PD | |
RT‐PCR markers | Albumin | – | – | + | + | + | ND |
αFP | – | + | + | + | + | + | |
CK‐8 | + | – | – | + | ND | – | |
CK‐18 | ND | – | – | – | – | – | |
α1‐AT | + | + | – | – | + | ND | |
G6Pase | – | ND | + | – | – | + | |
CYP 3A4 | – | – | ND | – | – | – | |
CYP 2B6 | – | – | ND | – | – | – | |
TAT | + | + | – | + | ND | – | |
TDO | ND | + | + | – | – | ND | |
HNF4 | ND | – | – | – | – | – | |
c‐Met | – | – | – | ND | + | + | |
PEPCK | + | ND | – | – | – | + | |
ICC markers | Albumin | – | – | + | + | + | + |
αFP | – | + | – | – | + | + | |
DPPIV | + | + | + | + | + | + | |
CK8 | – | – | – | – | – | – | |
CK18 | – | – | – | – | – | – | |
HepPar‐1 | – | – | – | – | – | – | |
Connexin‐32 | + | ND | ND | + | + | + | |
E‐cadherin | ND | + | + | ND | + | ND |
α1‐AT, α1‐antitrypsin; αFP, α‐foetoprotein; BM, bone marrow; CK, cytokeratin; CYP, cytochrome P450; DPPIV, dipeptidylpeptidase IV; G6Pase, glucose‐6‐phosphatase; HNF4, hepatic nuclear factor 4; ICC, immunocytochemistry; ND, not determined; P, passage; PD, population doublings; PEPCK, phosphoenolpyruvate carboxykinase; TAT, tyrosine aminotransferase; TDO, tryptophan‐2,3‐dioxygenase.