Table 4.
Estimation of liver engraftment level after cell transplantation into SCID mice
| Groups | Fibronectin | Albumin | αFP | CK18 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell clusters | Cells/ cluster | Isolated cells | Total cells | Cell clusters | Cells/ cluster | Isolated cells | Total cells | Cell clusters | Cells/ cluster | Isolated cells | Total cells | Cell clusters | Cells/ cluster | Isolated cells | Total cells | |
| A | 0.7 (0–2) | 28 (10–41) | 3.7 (0–15) | 16.5 (0–65) | 0 | 0 | 0 | 0 | 0.2 (0–1) | 9 (5–9) | 2 (0–8) | 4.2 (0–17) | 0 | 0 | 0 | 0 |
| B | 2.2 (0–5) | 11 (3–24) | 31.2 (0–67) | 50.5 (0–109) | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 (0–17) | 4.2 (0–17) | ND | ND | ND | ND |
| C | 5 (0–12) | 11.8 (2–33) | 6.7 (0–17) | 65.7 (0–201) | 6.7 (0–16) | 8.7 (2–32) | 2.7 (0–12) | 60.8 (0–163) | 5.3 (0–12) | 8.7 (2–38) | 8.7 (0–26) | 55 (0–195) | 2.2 (0–6) | 9.6 (3–24) | 4.3 (0–19) | 26.7 (0–51) |
| D | 0 | 0 | 0 | 0 | 3.5 (0–8) | 4.8 (2–12) | 17 (0–39) | 33.7 (0–70) | 1.2 (0–3) | 6.8 (3–12) | 5.2 (3–7) | 13.7 (5–22) | ND | ND | ND | ND |
| E | 0 | 0 | 0 | 0 | 5.7 (0–9) | 5.6 (2–23) | 42.7 (3–70) | 74.3 (3–109) | 7.7 (3–15) | 8.3 (2–35) | 6.3 (0–15) | 70.3 (34–133) | 3.3 (0–7) | 7.5 (2–31) | 4.7 (3–7) | 28.3 (4–71) |
Values present the number of cell clusters, cell content of clusters, amount of isolated staining cells and total amount of cells per section for each group of staining performed in the mouse livers (minimum sections per group = 6). Data are provided as mean with range value in brackets. αFP, α‐foetoprotein; CK18, cytokeratin 18; ND, not documented; SCID, severe combined immune deficiency.