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. 2013 Jul 21;46(4):416–424. doi: 10.1111/cpr.12044

Figure 3.

Figure 3

BMP ‐4 promoted PPAR ‐γ protein expression. (a) After 3 days adipogenic induction, immunofluorescence staining was performed with anti‐PPAR‐γ (green). Cell nuclei were counterstained with 4,6‐diamidino‐2‐phenylindole (blue). (b) Integral optical density (IOD) was measured to evaluate PPAR‐γ concentration. PPAR‐γ was condensed in nuclei, with significantly higher IOD in 50 ng/ml BMP‐4 groups than in the control group and 10 ng/ml BMP‐4 groups. However, PPAR‐γ IOD of the 10 ng/ml BMP‐4 groups was not statistically significant compared to the control group. P values <0.05 were considered statistically significant, as indicated by *. Magnification ×100.