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. 2008 Apr 15;41(3):492–505. doi: 10.1111/j.1365-2184.2008.00535.x

Figure 2.

Figure 2

mESC proliferation in response to BMP4, Wnt3a or BMP4 + Wnt3a over 6 days. To define the effects of BMP4 and Wnt3a, and interaction between BMP4 and Wnt3a on mESC proliferation, mESCs cultured on 0.1% gelatin with mESC medium in the absence of LIF were separately treated with 100 ng/mL BMP4, 100 ng/mL Wnt3a, 100 ng/mL BMP4 + 100 ng/mL Wnt3a, or none of them (the control group). From days 0–4, there was no statistical difference in cell numbers of the four groups. On day 5, cell number of the group BMP4 + Wnt3a (156 453 ± 19 893) (mean ± SEM) was significantly greater than that of the control (29 408 ± 10711), **P < 0.001, and also those of the groups BMP4 (50 380 ± 2281) and Wnt3a (61 771 ± 8354), *P < 0.01. Furthermore, on day 6, although the groups BMP4 (98 361 ± 7439) and Wnt3a (118 093 ± 9884) had more cells when compared to the control (37 528 ± 5119), *P < 0.01, the cell number of the group BMP4 + Wn3a (261 960 ± 14 530) was markedly more than those of the other groups (the groups BMP4, Wnt3a and control), **P < 0.001. Shown are representative data of n = 3 experiments. Error bars represent SEM.