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. 2011 Oct 13;44(6):582–590. doi: 10.1111/j.1365-2184.2011.00787.x

Figure 1.

Figure 1

 Generation and growth analysis of HeLa MIF‐knockdown cells. (a) MIF expression. HeLa parent, pSRP control and pSRP/MIF knockdown cells were harvested and subjected to western blotting analysis with anti‐MIF antibody. Western blotting for GAPDH was performed to ensure equal loading of protein samples. (b) Growth curve. HeLa pSRP control and pSRP/MIF knockdown cells were seeded in 6‐cm plates and cell number was counted at 0, 1, 2, 3, 4, 5 and 6 days. (c) Analysis of apoptosis. Cells under normal growth conditions were harvested and immuno‐labelled with anti‐annexin V antibody conjugated to Alexa fluor 488. Expression of annexin V on cell surfaces was revealed by flow cytometry as described in the Materials and methods section. (d) Cell morphology. All photographs of the cells are at the same scale.