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. 2019 Apr 18;85(9):e00106-19. doi: 10.1128/AEM.00106-19

FIG 2.

FIG 2

Species identification for strain HDXY-02 as B. gladioli. (A) Phylogenetic tree based on 16S-23S rRNA gene spacer sequences of Burkholderia species. (B) Phylogenetic tree based on partial 16S rRNA gene of Burkholderia species; Ralstonia solanacearum K60-1 was used as an outgroup. GenBank accession numbers are indicated in brackets. Bootstrap values of >50% are shown. (C) Analytical PCR with five pairs of primers, Bglu-F/toxA-R2 (a), Bgla-F/toxA-R2 (b), LP1/LP4 (c), GLA-F/GLA-R (d), and PG1/PG2 (e), in strain HDXY-02 (lanes 1 and 2) and water blank (lanes 3). M, DNA marker. The sizes of PCR products are indicated on the right. (D) Colony morphology of B. gladioli HDXY-02.