FIG 4.

¹³C-NMR analysis of the E140S reaction product from [U-¹³C]MVA-5-P. The partial areas of the NMR spectra around the signals of C-3 (A and B) and the other carbons (C and D) of [U-¹³C]MVA-5-P are shown. For the data shown in panels A and C, the sample from the negative-control reaction with water was used; for the data shown in panels B and D, the sample from the reaction with the E140S mutant was used. Asterisks shown in green and red indicate, respectively, the signals of [U-¹³C]MVA-5-P and the product.