. 2019 Apr 18;85(9):e00256-19. doi: 10.1128/AEM.00256-19

TABLE 3.

Primers used to construct plasmids in this study

Primer function and name	Sequence (5′–3′)
Mutation of TacM3K expression plasmid
TacM3K(E140A)-forward	CTCAGGGCGGTCTCCGCGAGCGCAGGCAGGAGC
TacM3K(E140A)-reverse	GCTCCTGCCTGCGCTCGCGGAGACCGCCCTGAG
TacM3K(E140S)-forward	CTCAGGGCGGTCTCCTCTAGCGCAGGCAGGAGC
TacM3K(E140S)-reverse	GCTCCTGCCTGCGCTAGAGGAGACCGCCCTGAG
TacM3K(E140G)-forward	CTCAGGGCGGTCTCCGGCAGCGCAGGCAGGAGC
TacM3K(E140G)-reverse	GCTCCTGCCTGCGCTGCCGGAGACCGCCCTGAG
Construction of artificial MVA pathway
Ma0602-forward	ATCCGAGCTCGGTACAAGGAGTTATATATGATTTCGTGTTCTGCCCCC
Ma0602-reverse	TATTTATATCTCCTTTCATTCTAACCTTAAGCCCTGTTC
Ma0603-forward	AAGGAGATATAAATAATGAATGCTTCAACCGAACCTG
Ma0603-reverse	GGATCCCCGGGTACCTCATACCCTTTTATCCGGACTGATC
P_tac-M3K-Fw1	AAAAGGTACCAATGAGCTGTTG
P_tac-M3K-Fw2	AAGGAGATTATATATGACCTACAGATCAATAGGATC
P_tac-M3K-Rv1	AAAAAAGCTTACTAGTTCACTCTGGTCTTCTATGCCAG
P_tac-M3K-Rv2	ATATAATCTCCTTCCACACATTATACGAGCCGGATGATTAATTGTCAACAGCTCATTGGTACCTTTT
Pto0478-Fw	AAAAACTAGTAAGGAGTTATATATGAACGATCTGAACGTATACGG
Pto0478-Rv	AAAAAAGCTTTTACAGTTCCTCGATAATAATTTTC
Construction of known MVA pathway
ScMVK-forward	GAATTCGAGCTCGGTACAGGAGGAAATAACCATGTCTCTGC
ScDMD-reverse	CTAGAGGATCCCCGGTTATTCCTTTGGTAGACCAGTCTTTG
Ta1305-forward	CGTTTTTTTGGGCTAGCGATGACCTACAGATCAATAGGATC
Ta1305-reverse	GAACATTATCCTGGAGTTCATACTAGTCTCTGGTCTTCTATGCC
Ta0762-forward	GCATAGAAGACCAGAGTGAAAGGAGACTAGTATGAACTCCAGGATAATGTTCATC
Ta0762-reverse	GGATCCCCGGGTACCGAGCTCGAATTCTCATCTCCTGTCCACAAATTTC
Pto0478-MP-forward	CAGGAGATGAGAATTCGAGCTCGGTACAGGAGGAGAATGAACGATCTGAACGTATACG
Pto0478-MP-reverse	GTCGACTCTAGAGGATCCCCGGGTACCTTACAGTTCCTCGATAATAATTTTC
Ta0103-forward	GGAACTGTAAGGTACCCGGGAAGAGGATGATGATACTGAAGATAGGCG
Ta0103-reverse	GCAGGTCGACTCTAGAGGATCCTCATCTTATCACCGTACCTATG
Ta0893(full)-forward	AAAAGAGCTCAGGAGGAGAATGGATACAGCCGGAATGGAAC
Ta0893(truncated)-forward	AAAAGAGCTCAGGAGGAGAATGGATATTGCAGAGCTTCACAGGG
Ta0893-reverse	AAAAGGTACCTCACAGGCGATCCTCTTCGTC