TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| Salmonella enterica SL1344 | Wild type, Strr | 46 |
| Salmonella enterica SR3203 | ΔptsN | 13 |
| Salmonella enterica SR4101 | pfur-lacZ (Kmr) | This work |
| Salmonella enterica SR4102 | pfur-lacZ (Kmr) ΔptsN | This work |
| Salmonella enterica SR4103 | fur-FLAG | This work |
| Salmonella enterica SR4104 | fur-FLAG ΔptsN | This work |
| Salmonella enterica SR4125 | pfhuA-lacZ (Kmr) | This work |
| Salmonella enterica SR4125 | pfhuA-lacZ (Kmr) ΔptsN | This work |
| Salmonella enterica SR4131 | pfhuA-lacZ (Kmr) Δfur | This work |
| Salmonella enterica SR4132 | pfhuA-lacZ (Kmr) Δfur ΔptsN | This work |
| Escherichia coli DH5α | F– supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 47 |
| Escherichia coli BTH101 | F– cya-99 araD139 galE15 galK16 rpsL1 (Strr) hsdR2 mcrA1 mcrB1 | 28 |
| Plasmids | ||
| pCP20 | reppSC101ts Apr Cmr FLP+ cI857+ | 41 |
| pCE70 | repR6K Kmr Cmr FRT tnpR lacZY | 43 |
| pKD3 | repR6K Apr FRT Cmr FRT | 41 |
| pKD13 | repR6K Apr FRT Kmr FRT | 41 |
| pKD46 | reppSC101ts Apr paraBAD γ β exo | 41 |
| pUHE21-2lacIq | reppMB1 Apr lacIq | 48 |
| pFur | reppMB1 Apr lacIq fur | 21 |
| pJJ37 | reppMB1 Apr lacIq ptsN-His6 | 13 |
| pJJ48 | reppMB1 Apr lacIq fur-His6 | This work |
| pKT25 | Kmr repp15A | 44 |
| pUT18 | Apr reppMB1 | 44 |
| pT25-ptsN | Kmr repp15A ptsN | 13 |
| pT25-ptsN(H73A) | Kmr repp15A ptsN(H73A) | 12 |
| pT25-zip | Kmr repp15A zip | 44 |
| pT18-fur | Apr reppMB1 fur | This work |
| pT18-zip | Apr reppMB1 zip | 44 |
a
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Strr, streptomycin resistance.