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Surfactin has an essential role in the in vitro antifungal activity of B. subtilis. (A and B) Absence of antifungal activity of an NCIB3610 isogenic strain deficient in surfactin production (ΔsrfA, strain DG560) (Table 1). (C) Growth of F. verticillioides in the absence or presence of the surfactin-deficient derivative (ΔsrfA) DG560 in LB broth with shaking at 28°C as described in Materials and Methods. The results of five representative experiments are shown.
