TABLE 3.
Primers and probes used for qPCR quantification of selected microorganisms
| Microorganisma | Primers and probeb | PCR protocol |
|---|---|---|
| Penicillium, Aspergillus, and Paecilomyces variotii | PenAsp1mgb (Taqman); PenAspR1, 5′-GCCCGCCGAAGCAAC-3′; PenAspF1, 5′-CGGAAGGATCATTACTGAGTG-3′; PenAspP1mgb, 5′-FAM-CCAACCTCCCACCCGTG-TAMRA-3′ | Activation, 94°C for 3 min; denaturation, 94°C for 15 s; annealing/extension, 60°C for 60 s; cycles, 40 |
| Aspergillus fumigatus and Neosartorya fischeri | Afumi (Taqman); AfumiR1, 5′-CCGTTGTTGAAAGTTTTAACTGATTAC-3′; AfumiF1, 5′-GCCCGCCGTTTCGAC-3′; AfumiP1, 5′-CCCGCCGAAGACCCCAACATG-3′ | Activation, 94°C for 3 min; denaturation, 94°C for 15 s; annealing/extension, 60°C for 60 s; cycles, 40 |
a
For all organisms, refer to http://www.epa.gov/microbes/moldtech.html.
b
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.