Figure 1.

miR‐375 suppresses porcine PSC proliferation. Cells were transfected with mimic, inhibitor and nonsense oligomer (NC group) of miR‐375. Non‐transfected cells were used as the blank control (control group). (a) BrdU incorporation assay of porcine pancreatic stem cells (PSCs) after treatment with the mimic and inhibitor of miR375, representative images of BrdU staining are shown. (b) Quantification and statistical analysis for (a). (c) Cell viability as measured with CCK‐8 assay. (d) The mRNA levels of cell proliferation markers including PCNA, C‐Myc, Cyclin A and Cyclin D1, as detected with qRT‐PCR. (e) Western blot analysis of C‐Myc and PCNA protein. (f) Quantification of results from (e). Image J (V1.48d) gradation analysis was carried out. (g) Cell cycle analysis. (h) Quantification of S‐phase cells as described in (g). Values are mean ± SD of triplicate experiments. *P < 0.05; **P < 0.01.