Table 1.
Primer sequences used for qRT‐PCR
Primers | Forward | Reverse | Tm (°C) |
---|---|---|---|
c‐Myc | 5′‐ctggtgggcgagatcatca‐3′ | 5′‐cactgccatgaatgatgttcc3′ | 54 |
PCNA | 5′‐agtggagaacttggaaatggaa‐3′ | 5′‐agtggagaacttggaaatggaa‐3′ | 58 |
Cyclin A | 5′‐tggctgtgaactacattga‐3′ | 5′‐acaaactctgctacttctgg‐3′ | 58 |
Cyclin D1 | 5′‐tgaactacctggaccgct‐3′ | 5′‐caggttccacttgagttgt‐3′ | 58 |
Insulin | 5′‐aagcgtggcatcgtggag‐3′ | 5′‐tcaggactttattgggtttgg‐3′ | 58 |
NeuroD1 | 5′‐agctcccatgtcttccacgta‐3′ | 5′‐gaagttgccgttgatgctga‐3′ | 58 |
Nkx 6.1 | 5′‐agacccactttttccggaca‐3′ | 5′‐ccaacgaataggccaaacga‐3′ | 58 |
miR‐375 | 5′‐acactccagctgggtttgttcgttcggctc‐3′ | 5′‐ctcaactggtgtcgtggagtcggcaattcagttgagtcacgcga‐3′ | 58 |
Pdk1 | 5′‐ggaacagcgcagtacgtttct‐3′ | 5′‐ctcgtttccagctcggaatgg‐3′ | 58 |
Pdx1 | 5′‐gagcccgaggagaacaagc‐3′ | 5′‐tgacagccagctccaccc‐3′ | 58 |
Mafa | 5′‐ttcagcaaggaggaggtcat‐3′ | 5′‐acaggtcccgctctttgg‐3′ | 58 |
Ngn3 | 5′‐gcgagttggcactgagca‐3′ | 5′‐aagctgtggtccgctatgc‐3′ | 58 |
18S | 5′‐gtggtgttgaggaaagcagaca‐3′ | 5′‐tgatcacacgttccacctcatc‐3′ | 58 |
β‐actin | 5′‐gcggcatccacgaaactac‐3′ | 5′‐tgatctccttctgcatcctgtc‐3′ | 58 |