Table 1.

Primer sequences used for RT‐PCR. The primers were constructed on the basis of published human sequences, and selected using version 1.5 of Primer Express software available from Applied Biosystems

Gene	Primer sequence	Gene	Primer sequence
Osteocalcin	F – gcagagtccagcaaaggtg	PDX1	F – ctgcctttcccatggatgaa
	R – atgtggtcagccaactcgtc		R – aagttcaacatgacagccagc
PPARgamma2	F – gctgaatccagagtccgctg	ABCG2	F – gcttgcaacaaccatgacgaa
	R – caaactcaaacttgggctcc		R – gccagttgtaggctcatccaa
MyoD	F – agcactacagcggcgact	PAX7	F – ctttgccgctaccaggagac
	R – gcgactcagaaggcacgtc		R – tcgatgctgtgtttggcct
Myf–5	F – cagtcctgtctggtccagaa	von Willebrand	F – tgtcaagigacgtgicagca
	R – gaactagaagcccctggag		R – gcagtagaaatcgtgcaacg
Myogenin	F – cagcgaaigcagctctcaca	Ve Cadherin	F – tgtctgtttgttgaggaccc
	R – agttgggcatggtttcatctg		R – aagtggtagaaaggctgctg
MRF4	F – ggctctcctttgtatccagg	CXCR4	F – aggtagcaaagtgacgccga
	R – cctlagccgttatcacgagc		R – aagtaccagtttgccacggc
Desmin	F – gatggaataccgacaccaga	Rex‐1	F – aacatgagccagcaactgaag
	R – ggtaggtggcaatctccaca		R – agaaatcatcccctccgagag
CK18	F – gctggagagcaaaatccgg	FGF‐4	F – ctactgcaacgtgggcatc
	R – cctccttgagagcctcgaict		R – acatgccggggtacttgtag
CK19	F – gaactccaggattgtcctgca	hTert	F – gagaacaagctgtttgcggg
	R – aaccaggcttcagcatccttc		R – ggcatctgaacaaaagccgt
alfa Feto Protein	F – ttccagaacctgtcacaagctg	Sox‐2	F – accagaaaaacagcccgga
	R – tctcctctgcaacagtgctcat		R – tcatgagcgtcttggttttcc
Albumin	F – ggttgatgtgatgtgcactgc	Runx‐1	F – tcactgtgatggctggcaat
	R – tcccttcatcccgaagttcat		R – ctgcatctgactctgaggctga
CYP1B1	F – gcctttatcctctctgcggaa	Oct‐4	F – acatgtgtaagctgcggcc
	R – acaaagctggagaagcgcat		R – gttgtgcatagtcgctgcttg
CYP2B6	F – atggaaaccgctggaaggi	GAPDH	F – gcttgtcatcaatggaaatccc
	R – gccccaggaaagiatttcaaga		R – tccacacccatgacgaacatg