Figure 3.

Hypoxia modulates expression of its sensitive transcription factor, HIF ‐1α, and induces expression of angiogenesis‐related growth factors after crosstalk between chondrocytes and osteoblasts. Expression of HIF‐1α, VEGF‐A/‐B, VE‐cadherin and PDGF genes in osteoblasts and chondrocytes measured at different oxygen tensions and from different culture methods. GAPDH and β‐ACTIN were used as reference genes. The product sizes are indicated in the left lane. The samples were taken at 1, 2, 3 and 5 days. The gels shown are representative of three different experiments (n = 3).