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. 2015 Mar 24;48(3):338–347. doi: 10.1111/cpr.12184

Figure 5.

Figure 5

Knockdown of endogenous Nedd4 expression induced lysosome membrane permeabilization. (a) DU145 cells stably expressing Nedd4‐shRNA2‐1#, shRNA2‐2# and control S‐shRNA were treated with 1.5 μm staurosporine for 24 h. LMP was detected by AO staining with visualization by fluorescence microscopy. Scale bar, 10 μm. (b, c) DU145 cells stably expressing Nedd4shRNA2‐1#, shRNA2‐2# and control S‐shRNA were treated with 1.5 μm staurosporine for 24 h. Release of cathepsin D into the cytosol and membranes were detected by western blotting.