Skip to main content
. 2007 Jul 16;40(4):532–549. doi: 10.1111/j.1365-2184.2007.00448.x

Figure 3.

Figure 3

pRB and p53 immunocytochemical staining of U2OS cells either silenced for both tumour suppressors (3a and 3c) or transfected with scrambled sequences (3b and 3d). Monoclonal antibodies versus total RB (3a and 3b) and versus p53 (3c and 3d) were used. Cells silenced for pRB showed very weakly stained nuclei (3a), whereas nuclei of cells transfected with the scrambled sequences appeared to be deeply stained (3b). After ActD treatment, cells silenced for p53 showed a very weak immuno‐staining (3c) whereas the intensity of the staining reaction was very high in cells transfected with the scrambled sequences (3d). Bar = 10 µm. (e) Western blot analysis of pRB and p53 after specific RNA interference. The expression of pRB is strongly reduced in single (RB1i) and double (TP53i/RB1i) silenced samples in comparison to samples transfected with scrambled sequences (scr1, scr2). In cell treated with ActD to stabilize p53, the expression of p53 is very low in single (TP53i) and double (TP53i/RB1i) silenced samples.