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. 2015 Feb 10;48(2):209–220. doi: 10.1111/cpr.12174

Figure 8.

Figure 8

Effects of STOX ‐1 on cell numbers and PCNA , cyclin E, cyclin A expression are dependent upon the AKT pathway. (a) Total cell numbers were determined by CCK‐8 assay after utricular epithelial cells were transfected with STOX1 over‐expression lentivirus or paired vector control, and exposed to STOX1 over‐expression lentivirus, in the presence of AKT inhibitor MK2206 (10 μm). (b) Protein levels of PCNA, cyclin E, cyclin A, p‐AKT and β‐actin were determined by western blotting. Western blotting analysis was performed with cell lysates from utricular epithelial cells after transfection with STOX1 over‐expression lentivirus or paired vector control, and exposed to STOX1 over‐expression lentivirus in the presence or absence of AKT inhibitor MK2206 (10 μm), respectively. Note that STOX1 over‐expression induced PCNA, cyclin E, cyclin A, p‐AKT expression relative to β‐actin. In contrast, STOX1 had no significant effect on PCNA, cyclin E, cyclin A, p‐AKT expression in the presence of MK2206. Three independent experiments were performed in triplicate for each sample. Results are expressed as means ± SD (n = 3). Asterisk indicates significant difference as determined by one‐way ANOVA (*P < 0.05, ***P < 0.001).