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. 2012 Jul 26;45(5):456–465. doi: 10.1111/j.1365-2184.2012.00834.x

Figure 1.

Figure 1

Expression and purification rHhAm175 in P . pastoris GS 115 (Mut+) cells. (a) PCR analysis of Pichia integrants. Amplification of rHhAm175 cDNA from isolated genomic DNA of 10 transformants (lane 1–10 represent 1–10# clone) and negative control clone of GS115 (Mut+) (lane 12). The recombinant vector was positive control (lane 11). (b) SDS–PAGE analysis of purified rHhAm175. Western blot analysis of rHhAm175 with (c) monoclonal antibody against hexa‐histidine (M‐His6), (d) a polyclonal antibody against 19 C‐terminal amino acids of human amelogenin (P‐C19) and (e) polyclonal antibody against human full‐length amelogenin (P‐FL191). Aggregates in the higher molecular weight region are marked by an asterisk (*).