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Figure 3.

Figure 3

Effect of cell passage number on the population doubling of MC3T3‐E1 cells. Passage 25 cells were initially seeded into T‐25 flasks. Every 4 days the cells in each flask were counted, subcultured (1.25 × 103/cm2) and the number of population doubling calculated. The data represent the mean of four to six samples ± the standard error of the mean. *Significance (P < 0.05).