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. 2010 Jun 29;43(4):405–417. doi: 10.1111/j.1365-2184.2010.00691.x

Figure 1.

Figure 1

  Isolation of spermatogonial stem cells (SSCs) from testicular tissues of obstructive azoospermic (OA) and non‐obstructive azoospermic (NOA) patients. (A) Seminiferous tubules of OA (normal spermatogenesis), NOA (maturation arrest at spermatocyte stage; MA) and NOA (Sertoli cell only; SOC) patients. (B) Testicular cell selection procedure. (a) Primary culture in non‐coated dishes. (b) Non‐attached cells, transferred to collagen‐coated dishes for 4 h. (c) CD9‐positive cells separated using MACS. (C) Separated cell numbers in each step per patient (×105). Testicular biopsy weight per patient; OA = 71 mg and NOA = 81 mg (D). Expression pattern of germ‐cell markers in testicular tissues from OA and NOA, before start of experiment. Oct‐4 (general stem cells); integrin α6 and integrin β1 (spermatogonia); c‐Kit (differentiating spermatogonia and spermatocytes); transition protein (TP)‐1 (spermatids). Bars = 50 μm.