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. 2010 Jun 29;43(4):405–417. doi: 10.1111/j.1365-2184.2010.00691.x

Figure 2.

Figure 2

  Photomicrograph of spermatogonial stem cells (SSCs) under exogenous feeder‐free culture conditions and their proliferation. (A) In vitro propagation of spermatogonial stem cells (SSCs). SSCs from obstructive azoospermia (OA, a–e) and non‐obstructive azoospermia (NOA, f–j.) (B) MTT assay of proliferating SSCs in in vitro culture. MTT assays to evaluate proliferation of P3 SSCs after culturing in vitro for 5 days. (C) Total cell number SSCs during long‐term in vitro culture. Proliferation of SSCs at each passage of long‐term culture (3∼130 days) Bar = 50 μm.