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. 2010 Jun 29;43(4):405–417. doi: 10.1111/j.1365-2184.2010.00691.x

Figure 6.

Figure 6

  In vitro differentiation into sperm lineage cells. (A) Localization of intra‐acrosomal protein (IAP) in human testis and mature spermatozoa. (B) Localization of peanut agglutinin (acrosomal granule‐specific protein) in human testis and mature spermatozoa. (C) IAP staining in putative round spermatids. Staining (upper figure) and frequency (lower figure) of FITC‐IAP stained cells during in vitro differentiation of SSCs. (D) PNA staining in putative round spermatids. Staining (upper figure) and frequency (lower figure) of TRITC‐PNA‐stained cells during in vitro differentiation of SSCs. OA, obstructive azoospermic; NOA, non‐obstructive azoospermia; yellow arrow head and arrows, indicate positive signal for IAP and PNA. **Significant difference (P < 0.05).