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. 2016 Feb 3;49(1):69–78. doi: 10.1111/cpr.12237

Figure 2.

Figure 2

Expression of miR‐497 and VEGF ‐A in CRC cell lines. Expression levels of miR‐497 and VEGF‐A were determined using quantitative RT‐PCR and were normalized against an endogenous control (U6RNA) or GAPDH. Data were analysed using a ΔΔct approach and expressed as either corresponding ratio (2−Δct) or fold change (2−ΔΔc). (a,b) Down‐regulation of miR‐497 was observed in the HCT8, LOVO, Ls‐174, HCT116 and HT29 cell lines. Normal human colorectal tissue was used as a control. (c,d) Expression of VEGF‐A was significantly up‐regulated in all these five CRC cell lines compared to control human colorectal tissues.