Figure 6.
Examples of chimerism and proliferation of donor bone marrow cells (BMCs) at day 28 in the spleen of mice given HgCl2 and infused with donor male BMCs 1 day later: (a) black arrows indicate donor‐derived BMCs (Y chromosome paint seen as brown nuclear signal) under bright field illumination, original magnification ×500; (b) the same field under reflected‐light dark‐field illumination, white arrows point to clusters of silver grains (3H‐thymidine labelling); (c) the images in (a) and (b) were combined to help to show silver grains over cells that are of donor BMC origin. (d), (e) and (f) are 2‐fold magnifications of the boxed area in (a). (g) Engrafted donor BMCs in the spleen demonstrated by a FISH method, broken arrows point to Y chromosomes (Y chromosome paint seen as green nuclear signal, ×630 and ×1000 inset).