Table 2.

 RT‐PCR primer sequences and product sizes

Gene	Primer sequence (5′→3′)	Accession number	Size (bp)	Annealing temp. (°C)
Oct3/4	agg cag gag cac gag tgg a cga agc ggc aga tgg ttg t	NM_001009178	264	58.3
Nanog	tct cct ccg cct tcc tct ttg cct ctg aaa cct atc ctt g	NM_001100781	204	53.1
Sox2	ggg ctc tgt ggt caa gtc tag tcg gca tca cgg ttt	NM_001109181	435	62.1
β‐actin	cca agg cca acc gcg aga aga tga c agg gta cat ggt ggt gcc gcc aga c	NM_031144	587	58