Skip to main content
. 2008 Jul 8;41(4):625–644. doi: 10.1111/j.1365-2184.2008.00543.x

Figure 5.

Figure 5

Confirmation of differentially expressed proteins in hTERT–hMSCs at PD 95 and PD 275 in comparison with primary hMSCs at PD 12, using RT‐PCR and Western blot analysis. Semiquantitative RT‐PCR analysis of genes shown to confirm differentially regulated protein/genes in hTERT–hMSCs. Equal aliquots of total RNA were reverse transcribed and amplified with primers specific for annexin A1, annexin A2, annexin V, GSTP1, RCN1, CCT6A, ACTG1, TUBA1B, p53 and prohibitin using GAPDH as a control. Western blot analysis showed differential regulation of annexin A1, annexin A2, annexin V, GSTP1, RTN1, CCT6A, ACTG1, TUBA1B, p53 and prohibitin using GAPDH as control. hMSCs, human mesenchymal stem cells; hTERT, human telomerase reverse transcriptase.