Table 2.
Search results of differently expressed proteins in hTERT‐hMSCs in comparison with primary hMSCs
| Spot no. | Reporter identifier | Density volume (hMSC at PD 12) | Density volume (hMSC–hTERT at PD 95) | Density volume (hMSC–hTERT at PD 275) | P‐value | Expression in hMSC–hTERT | Molecular weight (MW) (Da) | Isoelectric point (pI) | Type |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Actin, γ1 propeptide (ACTG1) | 356 ± 31 | 748 ± 27 | 854 ± 19 | < 0.05 | Up‐regulated | 42.08 | 5.3 | Structural protein |
| 2 | Reticulocalbin 1 (RCN1) | 458 ± 29 | 1067 ± 34 | 1878 ± 56 | < 0.01 | Up‐regulated | 38.890 | 4.86 | Ca2+ binding protein |
| 17 | ATP synthase, F1 complex (ATP5B) | 452 ± 32 | 1401 ± 36 | 1853 ± 73 | < 0.01 | Up‐regulated | 56.525 | 5.26 | Energy generation |
| 20 | Chaperonin‐containing T‐complex subunit 6 (CCT6A) | 585 ± 36 | 1872 ± 67 | 3335 ± 134 | < 0.05 | Up‐regulated | 58.444 | 6.25 | Protein folding |
| 22 | Coactosin‐like 1 (COTL1) | 420 ± 34 | 1932 ± 35 | 1974 ± 48 | < 0.01 | Up‐regulated | 16.049 | 5.54 | Antioxidant |
| 26 | Elongation factor TU (EFTU) | 356 ± 14 | 1602 ± 175 | 1531 ± 95 | < 0.01 | Up‐regulated | 49.851 | 7.26 | Transcription translation |
| 49 | Heat shock 60 kDa 1 (HSPD1) | 365 ± 24 | 1095 ± 46 | 1606 ± 45 | < 0.01 | Up‐regulated | 61.187 | 5.7 | Protein folding |
| 53 | IMP cyclohydrolase (ATIC) | 542 ± 34 | 1626 ± 56 | 1572 ± 43 | < 0.01 | Up‐regulated | 65.089 | 6.27 | Intermediary metabolism |
| 61 | Peroxiredoxin 6 (PRDX6) | 567 ± 56 | 2552 ± 135 | 2324 ± 156 | < 0.05 | Up‐regulated | 25.133 | 6.02 | Antioxidant |
| 62 | Peroxiredoxin 1 (PRDX1) | 463 ± 41 | 2417 ± 168 | 2084 ± 201 | < 0.05 | Up‐regulated | 22.3 | 8.27 | Antioxidant |
| 96 | Tubulin, alpha 1b (TUBA1B) | 621 ± 26 | 1242 ± 61 | 1428 ± 37 | < 0.01 | Up‐regulated | 50.804 | 4.94 | Structural protein |
| 10 | Aldo‐keto reductase family 1, member A1 (AKR1A1) | 2431 ± 41 | 729 ± 49 | 972 ± 53 | < 0.01 | Down‐regulated | 36.200 | 6.55 | Intermediary metabolism |
| 12 | Annexin A1 (ANXA1) | 4234 ± 289 | 889 ± 51 | 931 ± 27 | < 0.01 | Down‐regulated | 38.918 | 6.64 | Ca2+ binding protein |
| 13 | Annexin A2 (ANXA2) | 5347 ± 256 | 802 ± 44 | 1016 ± 48 | < 0.01 | Down‐regulated | 38.780 | 7.57 | Ca2+ binding protein |
| 14 | Annexin V (ANX V) | 5421 ± 356 | 1084 ± 33 | 976 ± 19 | < 0.01 | Down‐regulated | 35.971 | 4.94 | Ca2+ binding protein |
| 19 | Chloride intracellular channel 4 (CLIC4) | 3452 ± 134 | 518 ± 56 | 828 ± 64 | < 0.01 | Down‐regulated | 28.982 | 5.45 | Signal transduction |
| 39 | Glutathione S‐transferase P1 (GSTP1) | 1354 ± 123 | 542 ± 12 | 406 ± 17 | < 0.01 | Down‐regulated | 23.595 | 5.44 | Protein binding |
| 51 | Isocitrate dehydrogenase 1 (NADP+) | 3275 ± 253 | 753 ± 45 | 1048 ± 68 | < 0.01 | Down‐regulated | 46.915 | 6.53 | Intermediary metabolism |
| 94 | β‐Tropomyosin (TPM2) | 2356 ± 32 | 754 ± 75 | 518 ± 32 | < 0.05 | Down‐regulated | 29.980 | 4.66 | Structural protein |
| 100 | WD repeat domain 1 (WDR1) | 1456 ± 124 | 495 ± 34 | 408 ± 35 | < 0.01 | Down‐regulated | 66.836 | 6.17 | Structural protein |
hMSCs, human mesenchymal stem cells; hTERT, human telomerase reverse transcriptase.