Figure 2.

  Mithramycin suppresses Sp1 mRNA expression in human gingival fibroblasts. Trizol‐extracted RNA from 40% to 60% confluent HGF treated overnight with or without 100 nm of mithramycin (control, mith) was used for RT‐PCR analysis. Experiments were conducted on two independent occasions. Data shown are mean values obtained from four replicate samples of each experiment (Exp 1 and Exp 2). Fold decrease in Sp1 mRNA expression relative to GAPDH control is shown.