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Effect of DHK on proliferation of KSCs. Note that DHK significantly increased proliferation of KSCs via Akt and ERK pathways. Compared to control (a), DHK treatment (50 μm concentration, b) increased proliferation of KSCs and relative cell number was measured (c). Expression of phosphorylated Akt and ERK1/2 were detected by western blot analysis (d). DHK‐induced KSC proliferation was attenuated by specific Akt (LY294002, 5 μm) and ERK1/2 (U0126, 5 μm) inhibitors (e). **P < 0.01.
