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Serum induces retinoblastoma protein (pRb) phosphorylation in neonatal cardiomyocytes. Cells were stimulated with 5% serum (a) and were incubated for 2 days with 30 µm differentiation inducing factor 1 (DIF‐1) or 10 µm roscovitine (b). Fraction of phosphorylated pRb‐positive cells was counted. *indicates P < 0.05, Student's t‐test. Data represent the result of three independent experiments. A minimum of 100 cells was counted.
