Figure 4.

Inhibition of cyclin D impairs hypertrophic growth in neonatal cardiomyocytes. Average cell size in neonatal myocyte cultures after stimulation with 5% serum (a) or 0.5 µm angiotensin II (AII) (c) and were incubated for 3 days with 30 µm differentiation inducing factor 1 (DIF‐1) or 10 µm roscovitine (b) (d). Size of the cells (arbitrary units) under each condition was determined as described in the Material and Methods section. Error bars denote SD. *indicates P < 0.05, Student's t‐test. Data represent results of three independent experiments. A minimum of 50 cells was measured.