Figure 5.

DNcdk2 expression does not impair hypertrophic growth, but inhibits DNA synthesis in neonatal cardiomyocytes. Average cell size in neonatal myocyte cultures infected with dominant‐negative CDK2 (DNcdk2) or adenoviral vectors expressing mock (m.o.i. = 10) and incubated for 3 days in basal serum or with 5% serum (a). Size of the cells (arbitrary units) under each condition was determined as described in the Material and Methods section. Error bars denote SD. *indicates P < 0.05, Student's t‐test. Data represent results of three independent experiments. A minimum of 50 cells was measured. (b) Cells were infected as in (a) and 1 day after infection DNA synthesis was measured by ³H‐thymidine incorporation over a 24‐h period in cells treated with 5% serum. Data represent results of three independent experiments.