Table 1.
Antibodies for immunocytochemistry
| Antigen | First antibody | Vendor or reference | Second antibody b | Vendor | ||
|---|---|---|---|---|---|---|
| Source a | Dilution | Source | Dilution | |||
| TGFα | Rabbit p | 1 : 100 | Research & Diagnostic Ab | Goat | 1 : 160 | Sigma |
| bFGF | Rabbit p | 1 : 50 | Sigma | Goat | 1 : 160 | Sigma |
| Keratin 8 | Rat m | 1 : 40 | DSHB c | Rabbit | 1 : 20 | Vector |
| Keratin 14 | Mouse m | 1 : 20 | Neomarkers | Horse | 1 : 100 | Vector |
| Keratin 18 | Rabbit p | 1 : 25 | Ku, et al. (1995) | Goat | 1 : 100 | Sigma |
| Vimentin | Mouse m | 1 : 30 | Sigma | Horse | 1 : 100 | Vector |
| E‐cadherin | Rat m | 1 : 250 | Sigma | Rabbit | 1 : 20 | Vector |
| Epithelial membrane antigen | Mouse m | 0 | Dako | Primary FITC‐ conjugated | ||
a
m, monoclonal; p, polyclonal.
b
b All second‐stage antibodies were FITC‐conjugated.
c
c Developmental Studies Hybridoma Bank, University of Iowa.